Drug Susceptibility Testing for Mycobacterium tuberculosis Using Microtiter Plate
نویسندگان
چکیده
منابع مشابه
Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.
A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.
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Determination of the drug susceptibility of Mycobacterium tuberculosis by conventional methods using an agar-based medium may take 3 weeks or more to complete. On the other hand, results on positive cultures are generally available in 4 to 7 days with the radiometric (BACTEC, Johnston Laboratories, Towson, Md.) procedure. One disadvantage to the latter is the requirement to determine the quanti...
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In their recent article, Kristian Ängeby et al. (4) reported a novel test for drug susceptibility testing of Mycobacterium tuberculosis. However, it should be clarified that this method was previously published in 1989 (3) by Emil Kalfin, (National Institute for Lung Diseases, Sofia, Bulgaria) and Andreas Engibarov (National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria). It is n...
متن کامل[External quality assessment of drug susceptibility testing for Mycobacterium tuberculosis].
OBJECTIVE The Committee for Mycobacterial Examinations has planned and implemented the third external quality assessment of drug susceptibility testing for Mycobacterium tuberculosis to the hospital and private laboratories. METHOD The Committee delivered 20 M. tuberculosis strains, exactly pairs of 10 strains, which were evaluated and standardized for the drug resistance pattern in the WHO/ ...
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The drug susceptibility testing performance of a broth-based method with microscopic reading of bacillary growth, the microscopic observation drug susceptibility (MODS) assay, was compared to that of the reference 7H10 agar method of proportion by using 53 isolates of Mycobacterium tuberculosis from persons at risk for multidrug-resistant TB. For isoniazid (0.1 micro g/ml) and rifampin (2.0 mic...
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ژورنال
عنوان ژورنال: Tuberculosis and Respiratory Diseases
سال: 1990
ISSN: 1738-3536,2005-6184
DOI: 10.4046/trd.1990.37.3.278